14^th International Conference on Pharmaceutical Education and Practice October 28-28, 2022 Vancouver, Canada

Biography:

Mr.Harshkumar Brahmbhatt has completed his Master of Pharmacy from CHARUSAT University, Changa, Gujarat, India. He has 1 year and 7months of experience as Trainee Scientist II at Torrent Pharmaceuticals Ltd, Gandhinagar, India and from September 2019 onwards he is working as an Assistant Professor at School of Pharmacy, ITMBU, Vadodara, India. He has received grant from GUJCOST(a state government body) for the research work. He is director of Aryan crop Protection Pvt Ltd. based in Nadiad, Gujarat, India -A agricultural Products manufacturing company. In the past he has presented at several platforms including Boston International Conference.

Abstract:

Polysorbate 80 is a nonionic surfactant derived from polyethoxylated sorbitan. It is often used in the pharmaceutical formulation for solubility and stability of the product. An optimal concentration of polysorbate 80 is required for its effect. The methods reported for the determination of polysorbate 80 are based on HPLC separation with evaporative light scattering detection or charged aerosol detection. A simple, rapid and sensitive pectrofluorimetry method was developed for the determination of a non-ionic surfactant polyoxyethylene sorbitan monooleate (Polysorbate 80) from a pharmaceutical formulation. The proposed method is based on a fluorescence enhancement of the probe 4',5’- dibromo-2’,7’-dinitro-fluorescein disodium salt (Eosin B, 4µg/mL) in the presence of a surfactant (polysorbate 80). The fluorescence intensity was recorded using excitation wavelength (λex) at 545nm and emission wavelength (λem) at 580nm. The solutions were adjusted to pH 4.0 using a citric acid buffer. The calibration curves were obtained in the range of 16 µg/mL to 80 µg/mL of polysorbate 80 with a regression coefficient >0.99. The developed method was validated as per the ICH Q2 (R1) guideline in terms of linearity, precision, accuracy, robustness, detection limit and quantification and specificity. The limit of detection and limit of quantification for polysorbate 80 was found to be 2µg/mL and 16µg/mL, respectively. The developed method was successfully applied for the determination of polysorbate 80 in marketed ophthalmic solution and in-house prepared microemulsion containing polysorbate 80.

Biography:

Tamar Gilon has completed her PhD from The Hebrew University from The Department of Biochemstry and Molecular  Biology The Hebrew Universty Jerusalem. From the year 2008 Tamar has been a faculty member at The Department of Pharmaceutical Engineering, Azrieli College of Engineering, Jerusalem and part of the developing team that build a unique pharmaceutical engineering multidisciplinary B.Sc degree. Academic activity performed include; Head of the Center for Promotion of Teaching & Learning, Member of  the Departmental Pedagogical Committee, Tamar is an active researcher at the college in the field of fermentation of geneticly 

Abstract:

Biopharmaceutical drugs and biomanufacturing are a rapidly growing industry. Education and training for production and processing in these field are lagging. As part of the Pharmaceutical Engineering B.Sc. undergraduate program at Azrieli College of Engineering Jerusalem a hands on fourth year student laboratory training course was developed that centers on biotechnology methods and operating a bioreactor. This newly developed course applies pharmaceutical engineering knowledge, practical and theoretical, in the bioprocess and biopharmaceutical industry, including upstream optimization, fermentation and downstream process, based on the unique multidisciplinary curriculum of the Pharmaceutical Engineering degree. The aims of the course are to equip the pharmaceutical engineering students with the necessary knowledge and skills to function effectively within any area of the biopharmaceutical sector, with the adaptability to respond to the needs of a rapidly evolving industry and to facilitate the transition from academia to industry. At the core of the course, student preform a lab scale fermentation process for the production, purification and analysis of a recombinant active Tyrosinase enzyme. Tyrosinase can then be used to manufacture L-DOPA drug from L-tyrosine. The course is constructed of 6 hours of lectures following 22 hour of laboratory work. Student assessment are based on quizzes containing both theoretical and practical questions, laboratory work, learning skills and one cohesive report for the whole process, summarizing all the experiment data, results as well as calculation. 

Biography:

Ivan Kahwa completed his Msc.in Pharmacognosy and Medicine Science from Mbarara University of Science and Technology and has attended several trainings in natural products from different institutions. He is the director of KivanWoods Herbal Products and also a part time lecturer at Mbarara University of Science and Technology. He has published more than 10 papers in reputed journals

Abstract:

Artemisia vulgaris L.  is used for treatment of malaria in Western Uganda instead of Artemisia annua L. Until to date no scientific evaluation has been reported on whether the leaves of the two species have similar physical and chemical characters.The present study evaluated the botanical, physicochemical, and chemical characters of leaves of A. vulgaris L.  Macroscopy and microscopy studies, histochemical, physicochemical properties, and chemical parameters were carried out using standard procedures. Macroscopically, the leaves are dark green, with a sweet, strong and aromatic odor, pleasant, tangy and bitter taste. Microscopically, epidermis is amphistomatic, has anomocytic stomata on both adaxial and abaxial leaf surfaces and bicellular, biseriate glandular trichomes and unicellular T-shaped non-glandular trichomes. Histochemical studies showed the presence of cell wall materials and cell inclusions. Leaf constants revealed stomatal number (10.0), stomatal index (32.6), palisade ratio (7.8), vein islet number (15.0) and vein termination number (17.0). Physiochemically, moisture content (8.10 %), total ash (10.30 %), acid-insoluble ash (1.60 %), and sulphated ash (13.90 %), water-soluble ash (5.02%), alcohol-soluble extractive (9.73 %) and water-soluble extractive (19.9 %). Chemically, all the preliminary secondary plant metabolites were present except saponins and anthraquinones. Major fingerprints of extracts were showed at nine (9) Rf values.  Artemisinin concentration was 1.72% w/w and total flavonoid contents were 1.10 and 0.84 mg QE/g in diethyl ether and methanol extracts respectively. These findings are of importance in the establishing diagnostic features of A.vulgaris L. which could be used for identification and preparation of its monograph.

Biography:

Sarnai Sukhbaatar has completed a master's degree in 2015 from the Mongolian National University of Medical Sciences (MNUMS) and is now doctoral studies at MNUMS. She successfully defended clinical medicine with a dissertation on “Result of silymarin liver protection in anti-tuberculosis treatment”. She is fully capable of conducting clinical medicine experiments independently, well versing in clinical pharmacological research methods, and summarizing the results. Now her research area is adverse drug reactions.

Abstract:

Drug induced disorders incidence is increasing year by year and severe cutaneous adverse drug reactions (CADRs) are rare and life threating. The incidence of adverse effects is known higher in elderly people but in Mongolia, studies on drug have been used in elderly patients were treated in the National Dermatology Center of Mongolia (NDCM) have not yet been conducted.

The purpose of this study is to reveal the medicines affecting the development adverse reaction from 2013 to 2021 and aging groups’ specialty. 

We conducted a study on the medical histories who were hospitalized at the NDCM from 2013 to 2021. We used retrospective design in the medical histories with L23, L27, and L50-53 diagnosis (ICD classification) and were selected medical histories that were patients were above 55 years.  The age group classification was based on the “National Strategy for Population Ageing 2009-2030”. We analyzed total 170 elderly patients with CADRs (45.3%) were treated in that hospital during 2013-2021 and other patients dermatitis (approximately 55%) causes were food and chemical substances. In the 2013, geriatric CADRs cases were 20, in the 2021 were 31, therefore CADRs are likely to increase year by year.  The common offending drug groups were antimicrobials (40%), and central nervous system drugs (1.4%). In the old age groups CADRs cases were 89 (55%), and young old group 46 (28.7%). The common offending drug groups were antimicrobials and central nervous system drugs for elderly patients. The most common adverse drug reactions of old age group.

Biography:

Tamar Gilon has completed her PhD from The Hebrew University from The Department of Biochemstry and Molecular  Biology The Hebrew Universty Jerusalem. From the year 2008 Tamar has been a faculty member at The Department of Pharmaceutical Engineering, Azrieli College of Engineering, Jerusalem and part of the developing team that build a unique pharmaceutical engineering multidisciplinary B.Sc degree. Academic activity performed include; Head of the Center for Promotion of Teaching & Learning, Member of  the Departmental Pedagogical Committee, Tamar is an active researcher at the college in the field of fermentation of geneticly engineered bacteria for production of recombinant proteins

Abstract:

Biopharmaceutical drugs and biomanufacturing are a rapidly growing industry. Education and training for production and processing in these field are lagging. As part of the Pharmaceutical Engineering B.Sc. undergraduate program at Azrieli College of Engineering Jerusalem a hands on fourth year student laboratory training course was developed that centers on biotechnology methods and operating a bioreactor. This newly developed course applies pharmaceutical engineering knowledge, practical and theoretical, in the bioprocess and biopharmaceutical industry, including upstream optimization, fermentation and downstream process, based on the unique multidisciplinary curriculum of the Pharmaceutical Engineering degree. The aims of the course are to equip the pharmaceutical engineering students with the necessary knowledge and skills to function effectively within any area of the biopharmaceutical sector, with the adaptability to respond to the needs of a rapidly evolving industry and to facilitate the transition from academia to industry. At the core of the course, student preform a lab scale fermentation process for the production, purification and analysis of a recombinant active Tyrosinase enzyme. Tyrosinase can then be used to manufacture L-DOPA drug from L-tyrosine. The course is constructed of 6 hours of lectures following 22 hour of laboratory work. Student assessment are based on quizzes containing both theoretical and practical questions, laboratory work, learning skills and one cohesive report for the whole process, summarizing all the experiment data, results as well as calculation. 

Biography:

Ivan Kahwa completed his Msc.in Pharmacognosy and Medicine Science from Mbarara University of Science and Technology and has attended several trainings in natural products from different institutions. He is the director of KivanWoods Herbal Products and also a part time lecturer at Mbarara University of Science and Technology. He has published more than 10 papers in reputed journals

Abstract:

Artemisia vulgaris L.  is used for treatment of malaria in Western Uganda instead of Artemisia annua L. Until to date no scientific evaluation has been reported on whether the leaves of the two species have similar physical and chemical characters.The present study evaluated the botanical, physicochemical, and chemical characters of leaves of A. vulgaris L.  Macroscopy and microscopy studies, histochemical, physicochemical properties, and chemical parameters were carried out using standard procedures. Macroscopically, the leaves are dark green, with a sweet, strong and aromatic odor, pleasant, tangy and bitter taste. Microscopically, epidermis is amphistomatic, has anomocytic stomata on both adaxial and abaxial leaf surfaces and bicellular, biseriate glandular trichomes and unicellular T-shaped non-glandular trichomes. Histochemical studies showed the presence of cell wall materials and cell inclusions. Leaf constants revealed stomatal number (10.0), stomatal index (32.6), palisade ratio (7.8), vein islet number (15.0) and vein termination number (17.0). Physiochemically, moisture content (8.10 %), total ash (10.30 %), acid-insoluble ash (1.60 %), and sulphated ash (13.90 %), water-soluble ash (5.02%), alcohol-soluble extractive (9.73 %) and water-soluble extractive (19.9 %). Chemically, all the preliminary secondary plant metabolites were present except saponins and anthraquinones. Major fingerprints of extracts were showed at nine (9) Rf values.  Artemisinin concentration was 1.72% w/w and total flavonoid contents were 1.10 and 0.84 mg QE/g in diethyl ether and methanol extracts respectively. These findings are of importance in the establishing diagnostic features of A.vulgaris L. which could be used for identification and preparation of its monograph.